中文摘要：

目的观察1，25-二羟基维生素D3[1，25-（OH）2D3]对体外人牙周膜细胞（HPDLC）破骨细胞分化因子（ODF）mRNA及破骨细胞发生抑制因子（OCW）mRNA表达的影响，探讨正畸牙周组织改建的分子机制。方法以不同浓度的1，25-（OH），D3（0、1×10^-10、1×10^-8、1×10^-6mol／L）作用于体外培养的人牙周膜细胞，利用原位杂交染色及RT—PCR检测技术，检测不同浓度的1，25-（OH）2D3作用于体外培养人牙周膜细胞ODF、OCIF mRNA表达的强度变化。结果随1，25-（OH）2D3浓度的升高，人牙周膜细胞ODF mRNA的表达升高，而OCIF mRNA的表达降低。结论1，25-（OH）2现在体外可影响人牙周膜细胞ODF mRNA和OCIF mRNA的表达，从而通过调节ODF和OCIF相对含量的变化，影响牙周组织改建过程。

英文摘要：

Objective To clarify the molecular mechanism of periodontal tissue remodeling during orthodontic tooth movement by investigating the effects of 1,25-（OH）2D3 on the expression of ODF and OCIF in hmnan periodontal ligament cells （HPDLCs）. Methods HPDLCs were subjected to different doses of 1,25-（OH）eD3（0, 1 × 10^-10,1 × 10^-8, 1 ×10^-6 mol/L） for 24 hours in this experiment, mRNA expression of ODF and OCIF were detenninod by semi quantitative RT-PCR approach. Results The expression of ODF mRNA and increased significantly in a dose-dependent manner,and the expression of OCIF mRNA didn＇t decrease not significantly. Conclusion 1,25-（ OH）eD3 could adjust the proportion of expression of ODF to expression of OCW. Thus, the hone metabolism was adjusted.