中文摘要：

目的探讨钩藤碱和异钩藤碱对血管紧张素Ⅱ致血管平滑肌细胞凋亡的影响及其机制。方法建立血管紧张素Ⅱ诱导血管平滑肌细胞凋亡模型,通过倒置相差显微镜、激光共聚焦显微镜、流式细胞术和逆转录聚合酶链反应观察钩藤碱和异钩藤碱对细胞形态、细胞凋亡率、细胞[Ca^2＋]i浓度、Bcl-2和Bax蛋白表达与mRNA转录的影响。结果钩藤碱和异钩藤碱能诱导血管平滑肌细胞凋亡,提高细胞凋亡百分率,降低细胞[Ca^2＋]i浓度,下调Bcl-2蛋白表达、上调Bax蛋白表达,升高BaxmRNA/Bcl-2mRNA的比值。结论钩藤碱和异钩藤碱具有诱导血管平滑肌细胞凋亡的作用,其机制与降低细胞[Ca^2＋]i浓度、下调Bcl-2蛋白表达及mRNA转录、上调Bax蛋白表达及mRNA转录有关。

英文摘要：

Aim To study the effect of rhynchophylline and isorhynchophylline on the apoptosis of vascular smooth muscle cell （VSMC） induced by angiotensin Ⅱ （AngⅡ） its mechanism in rats. Methods The apoptosis model in VSMCs induced by angiotensin Ⅱ was established. The effect of rhynchophylline and isorhynchophylline on the cell morphology, apoptosis rate, [Ca2＋]i concentration, Bcl-2 and Bax protein expression and mRNA expression were observed by invert phase contrast microscope, flow cytometry and RT-PCR. Results The rhynchophylline and isorhynchophylline could induce the apoptosis of VSMCs, increase cell apoptosis rate, decrease cell [Ca2＋]i concentration and down regulate Bcl-2, Bax protein and mRNA expression. Conclusion The rhynchophylline and isorhynchophylline may induce the apoptosis of VSMC, whose mechanisms might be related to decreasing cell [Ca2＋]i concentration and down regulating Bcl-2 and Bax protein expression and mRNA expression.