中文摘要：

研究黑曲霉（Aspergillus niger）M-1菌株的α-葡萄糖苷酶基因在大肠杆菌中的克隆及表达。以M-1菌株的总RNA为模板，利用RT-PCR扩增α-葡萄糖转苷酶的cDNA，重组到Trc启动子控制下的表达载体pSE380中，构建重组质粒psE-atg，转入大肠杆菌BL21（DE3）进行IPTC诱导表达。初步研究表明：重组蛋白具有葡萄糖苷酶活性，最适pH为6．0，最适温度为45℃，金属离子Cu^2＋和Mn^2＋对酶活力有明显的促进作用。添加1．6mmol／L IPTC重组菌的诱导作用最大，在培养中添加麦芽糖，对重组菌产酶有显著的促进作用。

英文摘要：

The paper reports the research about cloning and expression of an α - glucosidase cDNA from Aspergillus niger in E. coli. cDNA was amplified by RT-PCR from total RNA of Aspergillus niger, and cloned into expression vector pSE380 which is controlled by Tre promoter, resultant pSE-atg was transformed to E. coli BL21（DE3 ） .The positive transformants were fermented in flasks and induced by IPTC. The recombinant α-transglucosidase activity amounted to 485.40U/g, and showed the optimal activity at pH 6.0 and 45℃. The research also found that the recombinant enzyme was obviously activated by Cu2^＋ and Mn2^＋ ion. Adding maltose in the substrate can induce the production of α - glucosidase by E. coil transformant.