中文摘要：

引言 过度使用化石燃料所造成的能源短缺和环境污染问题，已经对全球经济的可持续发展带来严重的挑战，因此研究开发可替代的清洁能源已成为科学界一项十分紧迫的任务。

英文摘要：

The pretreatment of bacteria source is one of the key factors affecting the efficiency of bio-hydrogen production.Prior to inoculation,cow dung compost as the natural microbial consortium is treated by following methods：（1）forced aeration combined adding sugar after infrared drying;（2）infrared drying;（3）infrared drying combined forced aeration;and （4）water immersion.The effects of the microbial community on bio-hydrogen production were investigated in a 20 L continuous stirred tank reactor （CSTR）.The results showed that the maximum hydrogen production ability,hydrogen content and the rate of hydrogen production was 387.6 ml H2·（g-sucrose）-1,55.5% and 0.5089 L H2·（L·h）-1 at fixed sucrose concentration of 10 g·L-1,operating pH of 5.0±0.1 and the pretreated bacteria source by method （1）,respectively.The contents of acetic acid and butyric acid were more than 75% of VFAs in the terminal liquid phase by-product.Furthermore,the image analysis by PCR-DGGE technology showed that there was district difference in the number of electrophoresis band and the strength.The sample,of which the bacteria source was treated by method （1）,exhibited two maximum illumination bands,which implies that there exist two kinds of dominant microbial community.