中文摘要：

在20L的半连续流发酵罐中，以牛粪堆肥为产氢菌源，按照玉米秸秆发酵浸出液的主要成分配制模拟废水，考察和分析了几个关键环境因素对发酵产氢的影响．结果表明，HBT、C／N、Fe^2＋浓度和模拟废水浓度对发酵产氢均有不同程度的影响．在本实验条件下，秸秆模拟废水的氢产量、氢浓度和产氢速率分别为11．80mol／kg、56％和8．81L／（L·d），底物转化率大于90％，废水中COD去除率为39．40％．在整个发酵产氢过程，液相主要发酵副产物为丁酸、乙酸和丙酸以及少量的乙醇和丁醇．

英文摘要：

Conversion of artificial corn stalk wastewater, which was prepared according to the main composition of the corn stalk fermentation lixivium, into bio-hydrogen gas by mixed culture was performed in a 20 L half-continuous flow fermenter. The influences of several environmental factors on the bio-hydrogen production, such as HRT, C/N ratio, Fe^2＋ and artificial corn stalk wastewater concentration were discussed in the tests. The experimental, results showed that HRT, C/N ratio, Fe^2＋ and artificial corn stalk wastewater concentration significantly affected the fermentation hydrogen production. The maximum H2 yield of 11.80 mol/kg, H2 concentration of 56% and hydrogen production rate of 8.81 L/（L· d） were obtained at HRT = 10 h, C/N = 100, Fe^2＋ concentration of 100 mg/L and substrate concentration of 12.5 g/L by mixed culture, respectively. In the fermentation hydrogen-producing process, the conversion efficiency of the substrate was more than 90%, and 39.40% of COD was removed from the reactor. The main by-products in the liquid phase were acetic acid, butyric acid, propionic acid and a little ethanol and butanol throughout this study.