中文摘要：

本实验克隆了果蝇与人源P-TEFb（positive transcription elongation factor）组成亚基CycT、CDK9 C末端分别带有Flag、HA标签的真核表达质粒，并在293细胞中验证了克隆所得质粒的有效表达。使用免疫共沉淀方法证实了两个种属 P-TEFb 组成亚基间结构的相似性；借助 HIV-luciferase与Rellina的双荧光报告系统证实了两个种属P-TEFb 组成亚基间功能上的相似性。

英文摘要：

In this study eukaryotic expression plasmid of Drosophila and human P-TEFb＇s subunit gene CDK9 and CycT was cloned,which each of the subunit has a Flag or HA tag in the C terminal respec-tively.Also was proved well express in the 2 9 3 Cell line.With the method of immunoprecipitation Dro-sophila P-TEFb and human P-TEFb subunits＇structure similarity was well confirmed.By means of dual-luciferase report system of HIV-luciferase and Rellina,P-TEFb subunits＇function similarity of the two species was also well affirmed.