中文摘要：

构建了带FLAG多肽标签的hHEXIMl（HMBA-inducible protein1）蛋白真核表达载体，在人胚胎肾细胞（human embryonic kidney 293 cells，HEK293 cells）中检测蛋白表达正确后，将该质粒转入小鼠的成纤维细胞（NIH3T3细胞）中，进行anti—FLAG的免疫沉淀（im—munoprecipitation，IP）实验．之后用半定量逆转录PCR（reverse transcriptase PCR，RT-PCR）的方法证明了hHEXIM1蛋白是可以和B2SINERNA相互作用的．并由此提出了关于B2SINERNA和HEXIM1蛋白共同参与调控基因表达调控的新假设．

英文摘要：

A FLAG peptide tagged hHEXIM1 protein expression plasmid was constructed. After expression ensured in HEK293 cells, the plasmid was tranfected into the NIH3T3 cells, then an anti-FLAG immunoprecipitation was carried out. The interaction between B2 SINE RNA and hHEXIM1 protein was figured out by semi-quantitative reverse transcriptase PCR （RT-PCR） followed. Given that, a hypothesis that B2 SINE RNA and HEXIM1 protein regulate the gene expression in the same pathway was proposed.