采用电子束辐射方法对铜绿微囊藻生长进行抑制和去除,通过呼吸速率及主要酶活性的变化,研究不同辐射剂量对藻细胞生理活动的影响。结果表明:电子束辐射使铜绿微囊藻受到严重迫害,呼吸速率及抗氧化酶活性在逆境胁迫条件下先增加后降低。3~5 kGy辐射迫害较为严重:藻细胞在辐射胁迫下引起抗氧化酶系统活性降低,体内活性氧产生和清除间的平衡被打破,产生氧化伤害,胞外多糖含量减少。低剂量的电子束辐射降低藻细胞生理活性,抑制藻细胞生长;4、5 kGy可使藻细胞短时间内失活,达到有效去除的目的。
Electron beam irradiation was applied to control and eliminate the growth of Microcystis aeruginosa,and the effects of different irradiation doses on the physiological activity of algae cell was studied through scanning the changes of the respiratory rate and antioxidant enzyme activity of the algae.The results show that the electron beam radiation makes Microcystis aeruginosa suffer severe persecution,and its respiratory rate and antioxidant enzyme activity increase firstly and then decrease under the condition of adverse circumstances.It also show that the irradiation doses of 3~5 kGy damage the algae most severely,its antioxidant enzyme activity decreases after irradiation intimidating,the equilibrium between the production and elimination of active oxygen in the algae cell is broken;then the oxidized damage comes,and the extracellular exopolysaccharide reduces.It was found that the radiation of low dose could reduce the algal physiological activity and control its growth,and the radiation doses of 3~5 kGy could make algae cell lose its activity in a short time and eliminate them efficiently.