中文摘要：

从鲫鱼的血样中分离淋巴细胞，以内分泌干扰物甲草胺和莠去津染毒，体外培养48h，采用四甲基偶氮唑盐（MTT）比色法测定两种除草剂对淋巴细胞生长活性的影响，并进行淋巴细胞的增殖实验，确定植物血凝素（PHA）刺激淋巴细胞增殖的适宜浓度，在此基础上开展两种除草剂对鲫鱼淋巴细胞增殖影响的研究。结果表明，甲草胺和莠去津对鲫鱼淋巴细胞生长活性的影响呈剂量-效应关系，其半数抑制浓度（IC50）分别为（27．7±7．6）、（97．6±26．4）μg／L；PHA的适宜刺激质量浓度是10．0μg／mL；两种除草剂对鲫鱼淋巴细胞增殖存在影响，当甲草胺质量浓度大于7μg／L、莠去津质量浓度大于50μg／L时，淋巴细胞增殖受到明显抑制，表明其对鲫鱼存在潜在的免疫毒性。

英文摘要：

The lymphocyte was separated from the blood sample of the fish Carassius auratus and cultured in vitro for 48 hours under exposure to alachlor and atrazine. The 3-（4,5-dimethylthiazol-2-yl）-2,5-diphenyltetrazolium bromide（MTT）method was used to determine the optimum concentration of phytohaemoagglutinin（PHA）in the pro- liferation test and the effects of alachlor and atrazine on lymphocyte growth and proliferation. The optimum PHA concentration was 10.0 μg/mL. Both alachor and atrazine affected lymphocyte growth, exhibiting the half inhibition concentration（IC50）of （27.7±7.6） μg/L and （97.64±26.4） μg/L,respectively. They also affected lymphocyte proliferation; the proliferation was significantly inhibited when the alachlor concentration was above 7 μg/L or the atrazine concentration was above 50 μg/L. The experimental results demonstrated that alachlor and atrazine have potential immunotoxicity to Carassius auratus.