中文摘要：

运用随机扩增多态性DNA（RAPD）技术在分子生物学水平上评价了低浓度阿特拉津长期暴露对鲫鱼DNA的影响．结果表明：0．006mg·L^-1以上浓度（试验浓度系列为0．006、0．023、0．094、0．375、1．5、3mg·L^-1）的阿特拉津持续暴露60d均会对鲫鱼的DNA产生影响；经筛选，确定有34条引物能够扩增出鲫鱼基因组DNA，其中有8条引物能检测出阿特拉津对鲫鱼基因组DNA影响的差异：各浓度组鲫鱼DNA的RAPD扩增产物条带均出现不同程度缺失；在较低浓度长期暴露下阿特拉津对鲫鱼基因组DNA的损伤无明显的剂量-效应关系，阿特拉津毒性在0．375mg·L^-1时突然增强，但这一趋势并未向更高浓度组延续（1．5mg·L^-1组表现出的毒性弱于0．375mg·L^-1组和3mg·L^-1组）．

英文摘要：

This article intends to study the effects of continuous exposure to low-concentration atrazine on the DNA of crucian （Carassius auratus ）. In this experiment, a random amplified polymorphic DNA （RAPD） technique is adopted to analyze the influences of continuous exposure （60d）to 0.006, 0.023, 0.094, 0.375, 1.5 and 3mg·L^-1 atrazine on the DNA of crucian. Results show that 8 primers among 34 primers used can produce different amplified fragments in crucian genomic DNA after 60d exposure. On the condition that the concentration of atrazine remains above 0.006mg·L^-1, it can influence crucian＇s DNA. Loss of different degrees of the amplified results is found in all treatments. There are no obvious dose-effect relationships between atrazine concentrations and DNA damages on condition of low concentration and long-term exposure, for the toxicity of 1.5mg·L^-1 atrazine is less than that of 0.375mg·L^-1 and 3mg·L^-1.