中文摘要：

对双孢蘑菇CGMCC No.0214及其基质降解能力退化菌株0214-3、0214-5进行了蛋白质双向电泳（2-DE或2D-PAGE）分析,发现了2个明显的、可重复的差异蛋白质,在退化菌株中分别为上调和下调表达,且2个退化菌株表现一致。根据质谱（MALDI-TOF/MS）分析和数据库检索结果,2个差异蛋白质初步鉴定为肌动蛋白和NADH脱氢酶铁硫蛋白3。

英文摘要：

The technique of two-dimensional electrophoresis （2-DE or 2D-PAGE） was used to analyze the protein expression differences between the wild type Agaricus bisporus CGMCC No.0214 and its substrate-decomposing ability degenerated strains 0214-3,0214-5.As a result,two proteins were found to be up- and down-expressed,respectively,with obvious and repeatable profiles in both degenerated strains when grown in a liquid culture.Based on the results of MALDI-TOF/MS analysis and database searching,these two proteins were identified as an actin and a putative NADH dehydrogenase iron-sulfur protein 3,respectively.The results of this study provided a marker on the proteomics level for further study of the molecular mechanism of substrate-decomposing ability degeneration in A.bisporus.