中文摘要：

目的 探讨氟西汀对小鼠肝脏药物代谢酶（Ces1d,Ces1e和CYP3A11）的作用及机制。方法 雄性C57BL/6N小鼠分为对照组（CON）、氟西汀低剂量组、氟西汀低剂量组和LPS组,分别腹腔注射等容积生理盐水、氟西汀10、20 mg/kg和LPS 2 mg/kg,连续3 d10提取肝脏组织蛋白,测定酶活性,Western Blot分析药物代谢酶（Ces1d、Ces1e、CYP3A11）、PXR和Stra13的表达。结果 氟西汀明显降低小鼠肝脏总水解酶活性以及羧酸酯酶（Ces1 d,Ces1e）的表达（P〈0.05、0.01）;明显降低小鼠肝脏CYP3A11活性以及氧化酶（CYP3A11）的表达（P〈0.05、0.01）;氟西汀能浓度依赖性降低调节药物代谢酶的核受体PXR的表达（P〈0.05、0.01）,同时增加转录因子Stra13的表达（P〈0.05、0.01）。结论 氟西汀降低药物代谢酶（Ces1d、Ces1e、CYP3A11）的表达及活性可能与其降低PXR表达和增加Stra13表达有关。

英文摘要：

Objective To study the effect of fluoxetin on drug metabolism enzymes in the mouse liver. Methods C57BL/6N mice were divided into four groups. The mice were injected intraperitoneally with equal volume of sa- line, 10,20 mg/kg fluoxetin, or 2 mg/kg LPS, respectively for three days. The livers were perfused to remove blood and Collected for enzyme assay and Western blot analysis. Results Fluoxetin represses the expression of Cesle, Cesl d and hydrolytic activity, and represses CYP3A11 and its activity in the mouse liver. Meanwhile, fluoxetin decreases PXR expression, which is an important regulator of drug metabolism enzymes, whereas in- creases Stral3 （DEC1） expression, which down -regulates CYP3All （CYP3A4）. Conclusion The repression of the drug metabolism enzymes（ Cesl d,Cesl e,CYP3A11 ） induced by fluoxetin in the mouse liver is related to the decrease of PXR and increase of Stra13 （DEC1）.