中文摘要：

选择小鼠脾脏和外周血T淋巴细胞作为研究对象,通过体外试验探讨黄芪多糖的免疫调节作用。用不同剂量的黄芪多糖作用于红细胞和T淋巴细胞共同培养体系,48h后,应用MTT比色法检测细胞的增殖活性,研究黄芪多糖对红细胞免疫调控功能的影响。结果表明,小鼠红细胞、植物血凝素（PHA）,单独作用对T淋巴细胞的增殖均有显著的促进作用,且红细胞对PHA激活的T淋巴细胞的增殖也有促进作用,但是二者没有明显的协同促进作用;黄芪多糖不能促进体外培养T淋巴细胞增殖,甚至还抑制PHA诱导的T淋巴细胞的增殖活性;但黄芪多糖可以协助红细胞对淋巴细胞的免疫调控功能,双方共同作用,引起淋巴细胞数目的增加和增强增殖功能。

英文摘要：

In this study,T-lymphocytes from mouse spleen and peripheral blood were used for in vitro experiments to explore the immunoregulatory effects of Astragalus polysaccharide（APS）.Co-cultures of red blood cells and T-lymphocytes were treated with different doses of APS for 48 h.MTT colorimetry was then used to determine cell proliferation in order to assess the effect of APS on the immunoregulatory function of red blood cells.Both mouse red blood cells and PHA significantly promoted T-lymphocyte proliferation when used alone.Red blood cells also promoted the proliferation of PHA-activated T-lymphocytes.However,the two did not show any significant synergistic effect.APS did not promote the proliferation of T-lymphocytes cultured in vitro and even inhibited the proliferation of PHA-activated T-lymphocytes.However,APS could aid the immunoregulatory effect of red blood cells on lymphocytes,and the combined effect of the two would enhance lymphocyte proliferation markedly.