中文摘要：

采用不连续垂直平板聚丙烯酰胺凝胶电泳技术,选取与隆线生长发育密切相关的5种同工酶:乳酸脱氢酶(LDH)、苹果酸脱氢酶(M DH)、酯酶(EST)、葡萄糖-6-磷酸脱氢酶(G-6-PDH)和细胞色素氧化酶(CO),比较分析了隆线溞在不种生殖状态下(孤雌溞、两性雌溞及雄溞)5种同工酶的表达差异.结果表明,隆线溞在3种生殖状态下,5种同工酶仅乳酸脱氢酶和酯酶呈现出酶带,这两种酶在孤雌溞和两性雌溞体内的表达变化基本一致,但乳酸脱氢酶雌溞、雄溞体内表达差异变化较大.其余3种同工酶的酶谱在隆线溞3种生殖状态下均不显著,可能是它们在隆线溞体内表达量很小,以至于活性较低.

英文摘要：

The patterns of five isozymic systems including lactate dehydrogenase,malate dehudrogenase,esterase,glucose-6-phospahate dehydrogenase and cytochrome oxidase in Daphnia(Ctenodaphnia) carinata,which were all correlative with the animal's growth and development,were investigated with polyacry lamidegel electrophoresis.The activities of five isozymes at three reproductive states(parthenogenetic female,sexual female and male) in Daphnia(Ctenodaphnia) carinata were been examined respectively and compared with ea...