中文摘要：

用福林酚法和复性电泳对红螯螯虾（Cherax quadricarinatus）雄性生殖系统的组织蛋白酶（Male Reproductive System Cathepsin,MRSC）进行了定性和定量分析,以探讨其在雄性生殖系统发育、精荚形成和精子释放过程中的作用。福林酚法测定结果显示：仔虾精巢蛋白酶比活力分别在pH6和pH10时出现2个高峰,而成虾精巢只在pH10时出现1个活力较高的峰。成虾雄性生殖系统的各部分在pH10～12的条件下有较强的蛋白酶比活力,于碱性缓冲液中浸泡有助于其后输精管内含物的溶解。复性电泳分离到4个蛋白酶条带,按分子量从大到小依次为：MRSC-A、MRSC-B、MRSC-C和MRSC-D。MRSC-A和MRSC-D广泛分布于雄性生殖系统各部分,其中MRSC-A为酸性蛋白酶,而MRSC-D是碱性蛋白酶;MRSC-C为酸性蛋白酶,只分布于中输精管;MRSC-B活性受pH值影响不大,仅存在于后输精管。在红螯螯虾雄性生殖系统的不同发育阶段,其组织蛋白酶的组成和活性不同。作为精荚的重要组成成分,组织蛋白酶与精荚排出体外后的硬化有直接关系,并促进随后精荚基质的水解以及精子的释放。

英文摘要：

Cathepsins are necessary for male reproductive system to maintain normal function.The Folin-Phenol reagent method and casein containing SDS-PAGE（sodium dodecyl sulfate polyacrylamide gel electrophoresis）were used for preliminary qualitative and quantitative analysis of the cathepsins in male reproductive system of Cherax quadricarinatus,in order to better understand the funtions of cathepsins during the development of male reproductive system,the formation and liquefaction of the spermatophore.The protease specific activities in premature and mature testis demonstrated different characters considering the pH value.At pH5-12,the activities of premature testis displayed two peaks respectively at pH 6 and 10;in mature testis only one peak of activity was observed at pH 10,of which the activity was higher those that in premature testis.In adults,the protease specific activities of vas deferens were higher than that in testis,among which anterior vas deferens had the highest activity.All parts of vas deferens showed high activities in alkaline pH range.Immersion in alkaline buffer of pH10-12 induced disassembly of the spermatophore matrix.As results of the casein containing SDS-PAGE assays,four bands with proteolytic activity were observed at pH6 and pH10,which were named MRSC（Male Reproductive System Cathepsin）-A,B,C and D coinciding with decreasing molecular weight.MRSC-A and D exhibited proteolytic activity within all tested parts of male reproductive system,including premature and mature testis as well as middle and posterior vas deferens of mature males.MRSC-A exhibited higher proteolytic activity in acidic pH range and the activities in middle and posterior vas deferens were higher than that in testis,by contraries MRSC-D was in alkaline pH range and it had higher activity in testis.Proteolytic activity of MRSC-C was detected exclusively in middle vas deferens,which was higher in acidic pH range.Activity of MRSC-B was exclusively in posterior vas deferens,which is slightly affected by pH.Cathepsin is an