中文摘要：

以禹州漏芦为原料,经沸水提取、乙醇沉淀、DEAE-Cellulose和SuperdexTM75凝胶柱层析分离纯化,得到一种水溶性的酸性均一多糖（EPS-2A）.采用单糖组成、甲基化及绝对构型等方法对其进行了结构解析.结果表明,该均一多糖主要由D-半乳糖醛酸组成,并含有少量的Rha和Ara.该均一多糖经不同条件硫酸衍生化后的衍生物（Sul-2A-1和Sul-2A-2）与阳性药肝素[CH50=（103.0±9.0）μg/mL（mean±SD,n=3）,CH50为出现50%溶血时的样品浓度]相比,均表现出很强的抗补体活性[Sul-2A-1的CH50=（74.1±4.6）μg/mL;Sul-2A-2的CH50=（35.7±2.8）μg/mL].

英文摘要：

To isolate and characterize the anti-complementary polysaccharide from the root of Echinops latifolius Tausch. , bioactivity-guided fractionation and purification was used to obtain the anti-complementary polysaccharide from the hot-water extract of the root of Echinops latifolius Tausch. The polysaccharide was characterized by various chemical and spectral analyses The anti-complementary activities were evaluated by hemolytic assay in vitro. The action targets were identified in the system with individual complement-depleted sera. A homogenous water-soluble polysaccharide （EPS-2A） was obtained from Echinops latifolius Tausch. , which average molecular weight was estimated to be 118000. A combination of monosaccharide composition, methylation and configuration analysis, as well as NMR spectroscopy, indicated that EPS-2A was poly-（ 1-4）- a-D-galactopyranosyluronic acid in which （87.8 ±0. 5 ）% of uronic acid existed as methylester. Two sulfated derivatives（ Sul-2A-1 and Sul-2A-2 ） from EPS-2A were prepared after sulfation with 1 ： 1 and 2 ： 1 of chlorosulfonic acid and pyridine, respectively. The anti-complementary assay showed that Sul-2A-1 and Sul-2A-2 demonstrated a stronger inhibitory effect [ CHs0 = （74. 1±4.6） μg/mL for Sul-2A-1 ; CH50 = （35.7±2. 8） μg/mL for Sul-2A-2] on the complement activation through the classic pathway, compared to that of heparin [ CHso = （103.0±9.0） μg/mL]. The results suggested that the sulfated derivatives Sul-2A-1 and Sul-2A-2 might be promising drug candidates in case of necessary therapeutic complement inhibition.