中文摘要：

为研究关白附多糖及其硫酸酯的经典途径抗补体活性，以关白附[Aconitumcoreanum（12v1．）Raipaics]为原料，经水提醇沉、DE-32、Superdex-200和Superdex-75凝胶柱分离纯化，得到1个均一的中性多糖KMPS-2A．采用高效凝胶渗透色谱法、甲基化、核磁共振和红外光谱等手段对KMPS-2A的结构进行了鉴定；采用氯磺酸吡啶法制备了多糖硫酸酯，并测定了多糖及硫酸酯的抗补体活性．结果表明，KMPS-2A的平均相对分子量为6．76×10%5，结构为α-1，6-D—Glc链接的线性多糖；在氯磺酸与吡啶的体积比为1．75：1．0时制备的多糖硫酸酯1．75B的取代度最高为1．79．碳核磁谱分析结果表明，硫酸基团先后取代C2，C3及C4位．该多糖硫酸酯的抗补体活性与其硫酸基团取代度呈现一定的相关性，多糖硫酸酯1．75B的经典途径抗补体活性优于阳性对照药肝素，表明其具有开发成为补体抑制剂的潜力．

英文摘要：

In order to study anti-complement activity in the classical pathway of Korean Mondshood root and its sulfated polysaccharides, a homogeneous polysaccharide （KMPS-2A） was gained from the Korean Mondshood root[ Aconitum coreanum （Levl.） Raipaics] by water extract and alcohol precipitate via DE-32, Super- dex-200 and Superdex-75 gel filtration. Its structure was investigated by high performance gel permeation chromatography, methylation analysis, NMR and IR spectrometry. The results showed that the average molecular weights （Mw） of the KMPS-2A was 676000 and it was consisted of a repeating structure as [ α-1,6-D-Glc ] n- Sulfated derivatives were gained by chlorosulfonic acid-pyridine method and it was found that the sulfated de- rivatives had the highest degree of substitution （ 1. 79） when the volume ratio of chlorosulfonic acid and pyridine was 1.75 ： 1.0. It was shown that sulfated polysaccharide 1.75B had a better anti-complementary activity. C2, C3 and C4 were substituted by sulfate groups. A certain correlation was presented between the degree of substitution and the anticomplementary activity of sulfated derivatives. Anticomplementary activity of sulfated derivative 1.75B was better than the heparin group in the classical pathway, which suggested the potential activity of the complement inhibitors.