中文摘要：

本研究通过优化小鼠白介素24（mIL-24）基因序列，构建以Fc-tag为标签的mIL-24-Fc融合蛋白．对CH0进行MTX分级加压筛选，获得了稳定、高表达mIL-24-Fc蛋白的阳性克隆，同时优化了CHO细胞无血清驯化与稳定表达蛋白体系．经proteinA亲和层析纯化mIL-24-Fc蛋白并利用APbinding实验检测生物学活性后，小鼠皮内注射mIL-24-Fc蛋白和伤口愈合实验证明：注射mIL-24-Fc蛋白后，小鼠表皮产生2～3层细胞增生，伤口愈合速度加快．

英文摘要：

In this study, the mIL-24-Fc fusion protein tagged with Fc-tag was constructed by optimizing the sequence of murine interleukin 24 （mIL-24） gene. The stable and high expression of mIL-24-Fc protein was screened at different concentrations of MTX, and the serum-free cell culture technique and stable expression system of CHO cells were optimized. MIL-24-Fc fusion protein was purified by protein A affinity chromatography and confirmed by AP binding assay. The result showed that after intradermal injections of mouse, a large number of keratinocytes showed two to three proliferation and in wound-healing process, epidermal hyperplasia was promoted and wound healing process was accelerated by mIL-24-Fc.