中文摘要：

为研究调节性T细胞在喉鳞状细胞癌（laryngeal squamous cell carcinoma,LSCC）、发展中的变化及其参与疾病进展的作用机制,收集2010～2011年上海市五官科医院收治的50例LSCC患者的肿瘤组织和外周血,应用流式细胞术检测CD4＋CD25＋Foxp3＋Treg细胞及趋化因子受体CCR6的表达变化,Real-time PCR法检测转录因子Foxp3以及细胞因子mRNA的表达量。结果发现：LSCC患者外周血中CD4＋CD25＋Foxp3＋Treg的百分比较正常人显著增加,并与临床分期相关;CD4＋CD25＋CCR6＋Treg Foxp3的表达,以及肿瘤组织Foxp3mRNA的表达皆明显高于对照组,且与临床分期、淋巴结转移相关。同时发现,LSCC患者外周血中TGF-β和IL-10mRNA的检出水平分别高于对照组,但IFN-γ、IL-2、IL-12mRNA的水平低于对照组。提示此类Foxp3＋Treg属于一类诱导性T抑制细胞（Foxp3＋iTreg）,可通过产生IL-10和TGF-β抑制LSCC患者的细胞免疫功能。Foxp3的检测可能对判断LSCC的预后有一定价值。

英文摘要：

To evaluate the correlation between regulatory T cells and laryngeal squamous cells carcinoma（LSCC）,peripheral blood samples were collected from 50 patients to determine the percentage of CD4＋C25＋Foxp3＋Treg cells by flow cytometry and the cytokine levels by real-time PCR.It was observed that not only the percentage of CD4＋C25＋Foxp3＋ Treg in peripheral CD4＋T cells of LSCC patients but also the positive ratio of Foxp3 in CD4＋CD25＋CCR6＋Tregs were much higher than those in healthy persons.By using real-time PCR,it was showed that the expression level of Foxp3 mRNA correlated well with clinical stages of LSCC and that the detectable levels of TGF-β and IL-10 mRNA were higher,and those of IL-2,IL-12 and IFN-γ mRNA were lower,in the patients when compared with those in healthy control.This suggests an involvement of the inducible regulatory T subset（Foxp3＋iTreg） in the pathogenesis of LSCC.The increased percentage of the Foxp3＋Tregs and the up-regulation of Foxp3 expression on CCR6＋Treg in LSCC patients,therefore,may account for the down-regulation of anti-tumor immunity in the patients,which may be valuable for assessment of prognosis in LSCC treatment.