中文摘要：

目的 探讨白细胞介素10（IL-10）启动子区-1082/-819/-592位点的多态性及其在血浆中的浓度与声带白斑发生的关系.方法 选择2012年10月至2013年2月61例声带白斑患者和119名正常人作对照研究,采用核苷酸直接测序法检测样本的IL-10基因位点（-1082G/A,-819C/T和-592C/A）的基因分型,用酶联免疫吸附法（ELISA）检测IL-10的血浆浓度.结果 吸烟和饮酒增加了罹患声带白斑的风险（OR为4.73和5.70,95％ CI为2.4 ～9.1;2.9～11.2,P＜0.01）.声带白斑患者在IL-10的-592和-819位点比对照组有更高的AC突变频率（OR=1.93,95％ CI为0.97～ 3.81,P=0.05）,在-1082位点有更高的AG突变频率（OR =2.14,95％ CI为0.87 ～5.27,P=0.092）;声带白斑患者的血浆IL-10质量浓度为（21.6 ±0.5）pg/ml（（x）±s）,对照组为（19.0±1.1） pg/ml,两者比较差异有统计学意义（t=2.08,P=0.043）.基因型中含有G基因的单倍体患者的血浆IL-10质量浓度（24.3±5.7）pg/ml,比正常基因型单倍体患者IL-10的浓度高（19.9 ±4.7） pg/ml,两者比较差异有统计学意义（t=-2.64,P=0.008）.结论 IL-10启动子区-1082/-819/-592多态性及血浆中高浓度的IL-10与声带白斑的发生有关,同时,突变基因型与IL-10血浆浓度有关.

英文摘要：

Objective To investigated the association between interleukin-10（ IL-10）-1082/-819/- 592 promoter polymorphism, plasma IL-10 levels and risk of vocal leukoplakia. Methods A case-control study of 61 patients with vocal cords leukoplakia and 119 healthy subjects were performed. Genotypes for the IL-10 gene （ IL-10 -1082 G/A, -819 C/T and -592 C/A） were determined using pyrosequencing and plasma IL-10 levels were analyzed by ELISA. Results Smoking and alcohol consumption increased the risk of vocal leukoplakia （ OR = 4.73, 95% CI：2. d - 9. 1 ; OR = 5.70, 95% CI：2. 9 - 11.2, P 〈 0. 01 ）. Patients with vocal cord leukoplakia had significantly higher frequencies of AC at -592 and -819 （ OR = 1.93,95% CI： 0.97-3.81,P=0.05） and AG at -1082 （ OR = 2.14 , 95%CI：0.87-5.27, P=0.092） than control. Vocal leukoplakiapatients had higher concentration of plasma IL-10 （21.6 ＋ 0. 5 ） pg/ml （x ± s） than controls （ 19. 0± 1.1 ） pg/ml（ t = 2.08, P = 0. 043 ） and the haplotype containing the G allele was associated with higher plasma IL-10 concentrations （ 24. 3 ±5.7 ） pg/ml compared with the ATA haplotype （ 19.9± 4. 7 ） pg/ml（t = -2. 64, P = 0. 008）. Conclusions IL-10-1082/-819/-592 promoter polymorphism and high concentration of plasma IL-10 are associated with vocal cord leukoplakia, and the concentration of plasma IL-10 is associated with the genotypes of IL-10 promoter polymorphism.