中文摘要：

目的：观察重组人粒细胞集落刺激因子（rhG-SF）与血管内皮生长因子（VEGF）联合应用，对快速老化模型小鼠P10（SAM—P10）认知功能的改善作用。方法：选用72只SAM—P10为实验动物，随机分为对照组、rhG-CSF治疗组（单治组）、rhG-CSF与VEGF联合治疗组（联合组），每组24只。对照组给予生理盐水，单治组皮下注射rhG-CSF100two／（kg·d），连续7d。联合组首先腹腔注射VEGF50μg／（kg·d），连续3d，随后皮下注射rhG-CSF 50μg（ks·d），连续7d。并于给药前及给药后第7、14、28、56d进行Morris水迷宫测试，观察其认知功能的变化。结果：对照组小鼠学习记忆能力下降，单治组和联合组均较对照组逃避潜伏期缩短，目的象限游泳距离百分比增加，且联合组优于单治组（P〈0．05）。结论：rhG-CSF与VEGF联合应用能改善SAM—P10的认知障碍。

英文摘要：

Objective： To observe the effects of rhG-CSF and VEGF on cognition disorders of senescence accelerated mouse P10 （SAM-P10）. Methods： Seventy-two SAM-P10 were randomly divided into a control group, rhG- CSF group and a rhG-CSF combined with VEGF group. Mice in the rhG-CSF group were subjected to 100 pg/（kg-d） rhG-CSF subcutaneouslly for 7 days, while mice in the rhG-CSF combined with VEGF group were first subjected to 50 μg/（kg· d） VEGF intraperitoneally for 3 days and then 50 μg/（kg· d） rhG-CSF subcutaeneouslly for 7 days. Mice in the control group were subjected to normal saline. The Morris water maze test was applied before the administration and on the 7th, 14th, 28th, and 56th day after the administration to observe the changes of the cognitive ability. Results： The ability of learning and memory declined in the control group. Compared with the control group, the escaping latency declined and the percentage of swimming distance in the target quadrant increased both in the rhG-CSF group and in the rhG-CSF combined with VEGF group. Moreover, the above items were better in the rhG-CSF combined with VEGF group than in the rhG-CSF group（ P 〈 0.05）. Conclusion： rhG-CSF combined with VEGF is able to improve cognition disorders of SAM-P10.