中文摘要：

目的建立大鼠血浆中阿霉素浓度的液相色谱-串联质谱（LC—MS／MS）测定方法。方法色谱柱：Venusil ASB C18（150mm×4．6mm，5μm），流动相：乙腈-5mmol·L“乙酸铵-甲酸（体积比为35．0：65．0：0．5），采用沉淀蛋白法，以多反应监测（multiplereactionmonitoring，MRM）扫描方式检测，测定大鼠尾静脉注射阿霉素纳米胶束溶液后血浆中药物浓度。结果血浆中阿霉素质量浓度在1～1000μg·L-1内线性关系良好，r=0．9960；日内和日间精密度RSD≤13．2％；阿霉素的平均提取回收率为89．7％-95．7％；阿霉素在大鼠血浆中主要药动学参数为t1/2（30．5±5．2）h，ρmax（954．3±80．6）μg·L-1，AUC0-∞／（290．2±40．4）μg·h·L-1。结论该方法适用于阿霉素在大鼠体内药动学的研究。

英文摘要：

Objective To develop a liquid chromatography-tandem mass spectrometry （LC-MS/MS） method for the determination of adriamycin and use the mathod to quantify adriamycin in rat plasma. Methods The plasma samples were deproteinated with acetonitrile,and separation was performed on a reversed-phase Venusil ASB C18 column（ 150 mm ×4. 6 mm,5μm）. The mobile phase contained acetonitrile -5 mmol.L-1 ammonium acetate-formic acid （ V： V： V = 35.0： 65.0： 0. 5 ）. Detection was carried out by multiple reaction monitoring. Adriamycin nanometer micelle was used in rats by inject caudal vein. Results The method has a linear calibration curve over the range of 1- 1 000 μg. L - 1 （ r = 0. 996 0）. The intra-day and inter-day accuracy and precision were within 13.2% for the analyte. The average recoveries were between 89.7%- 95.7%. Main pharmacokinetic parameters were as follows： tl/2 （ 30. 5 ± 5.2 ） h,max （ 954. 3 ± 80. 6 ） .g. L - 1, AUC0-∞ / （290. 2 ± 40. 4 ） μg. h. L - 1, respectively. Conclusions It is a rapid, sensitive, and specific analytic method. The proposed method can be appied for the identification and quantification in pharmacoki- netic studies of adriamycin in rat plasma.