目的探讨US-2030流式尿沉渣分析仪对胸腔积液有形成分的检测价值。方法采集胸腔积液标本110例。对US-2030流式尿沉渣分析仪检测胸腔积液有形成分的准确度、精密度、携带污染率、稀释线性进行分析,并与手工法检测结果比对分析。结果仪器法计数胸腔积液红细胞的准确度达到98.5%,精密度良好,携带污染率低,稀释线性良好。与手工法比较,仪器法在各量级胸腔积液红、白细胞计数差异均无统计学意义;应用仪器法尚发现27例胸腔积液标本小圆上皮细胞增高,经证实其中25例发现癌细胞。结论仪器法应用于胸腔积液有形成分的检测,具有不可忽视的临床辅助诊断价值,但仪器法不能完全取代手工法,对胸腔积液性质的确定仍需结合直接镜检、染色镜检、生化、培养等检测结果综合判定。
Objective To explore the clinical value of US-2030 type flow cytometer urine sediment analyzer in measurement of formed elements in pleural fluid.Methods Total 100 cases of pleural fluid samples were collected and detected by applying US-2030 type flow cytometer urine sediment analyzer and the manual method.The formed elements in pleural fluid were analyzed and compared about accuracy,precision,carry-over rate and dilution linearity between instrumental method and manual method.Results Using US-2030 type flow cytometer urine sediment analyzer,the accuracy of pleural fluid RBC count accounted for 98.5%,and the instrumental method had ideal precision and dilution linearity and low carry-over rate.There were no statistical differences in different degree of pleural fluid RBC count and WBC count between instrumental method and manual method.Using instrumental method,we found increased small round epithelial cells,among which 25 cases were confirmed as cancer cells.Conclusion The instrumental method has an important value in measurement of formed elements in pleural fluid,but it can not substitute the manual method.Property of pleural fluid is identified by combined results of direct test under microscope,microscopic observation after staining,biochemical examination and pleural fluid culture.