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Proteome changes in the plasma ofPapilio xuthus (Lepidoptera: Papilionidae): effect of parasitization by the endoparasitic wasp Pteromalus puparum (Hymenoptera: Pteromalidae)
  • ISSN号:1673-1581
  • 期刊名称:《浙江大学学报:B卷英文版》
  • 时间:0
  • 分类:S476.3[农业科学—农业昆虫与害虫防治;农业科学—植物保护] Q51[生物学—生物化学]
  • 作者机构:[1]State Key Laboratory of Rice Biology, Institute of Insect Sciences, Zhejiang University, Hangzhou 310029, China
  • 相关基金:Project supported by the National Basic Research Program (973) of China (No. 2006CB 102005), the National Natural Science Foundation of China (Nos. 30571251 and 30170626), the Program for New Century Excellent Talents in University of the Ministry of Education of China (No. NCET-05-0513), and the Innovation Research Team Program of the Ministry of Education of China (No. IRT0535)ACKNOWLEDGEMENT We are grateful to the Research Center for Proteome Analysis, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, China, for valuable help with proteomic mass spectrometry analyses.
中文摘要:

尽管 parasitoid 主人相互作用的生物化学的解剖正在变得很好描绘了,有关他们的分子的知识是最小的。为了理解对 parasitoid 的主人免疫者反应的分子的基地,攻击,我们探索了 Papilio xuthus 的反应用 proteomic 由 endoparasitic 黄蜂 Pteromalus 小狗海芋属植物寄生于途径。由检验血浆蛋白质的微分表达式在寄生于并且 unparasitized 主机蛹由二维(2D ) 电气泳动, 16 蛋白质被发现与 unparasitized 控制样品相比在与 parasitization 的关系变化。所有被结合的集体 spectrometry 他们提交到鉴定数据库搜索。调制蛋白质被发现掉进下列功能的组:体液或细胞的免疫, detoxification,精力新陈代谢,和其它。这研究贡献卓见进在 parasitoids 和他们的主人昆虫之间的关系的分子的机制。

英文摘要:

Although the biochemical dissection of parasitoid-host interactions is becoming well characterized, the molecular knowledge concerning them is minimal. In order to understand the molecular bases of the host immune response to parasitoid attack, we explored the response of Papilio xuthus parasitized by the endoparasitic wasp Pteromalus puparum using proteomic approach. By examining the differential expression of plasma proteins in the parasitized and unparasitized host pupae by two-dimensional (2D) electrophoresis, 16 proteins were found to vary in relation to parasitization compared with unparasitized control samples. All of them were submitted to identification by mass spectrometry coupled with a database search. The modulated proteins were found to fall into the following functional groups: humoral or cellular immunity, detoxification, energy metabolism, and others. This study contributes insights into the molecular mechanism of the relationships between parasitoids and their host insects.

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期刊信息
  • 《浙江大学学报:B卷英文版》
  • 中国科技核心期刊
  • 主管单位:
  • 主办单位:浙江大学
  • 主编:
  • 地址:杭州玉古路20号,浙江大学学报《英文版》编辑部
  • 邮编:310027
  • 邮箱:jzus@zju.edu.cn
  • 电话:0571-87952276 87952331
  • 国际标准刊号:ISSN:1673-1581
  • 国内统一刊号:ISSN:33-1356/Q
  • 邮发代号:
  • 获奖情况:
  • 国内外数据库收录:
  • 被引量:323