中文摘要：

由 endoparasitoid 黄蜂 Pteromalus puparum 的寄生状态在 Pieris rapae 的血浆蛋白质引起改变。用一条 proteomic 途径的血浆蛋白质的分析证明七蛋白质是在 24-h 寄生状态以后在主人蛹表示的差别。他们是像假面舞会的丝氨酸朊酶相当或相同的事物(MSPH ) ， enolase (Eno ) ， bilin 有约束力的蛋白质(BBP ) ，想象的圆盘生长因素(IDGF ) ，鸟氨酸 decarboxylase (ODC ) ，细胞的 retinoic 酸绑定蛋白质(CRABP ) ，和未知的相当或相同的事物工作蛋白质。MSPH， Eno，和 BBP 的完整的长度 cDNA 序列成功地用 cDNA 结束聚合酶链反应(RACE-PCR ) 的快速的扩大被克隆。反向的transcriptase聚合酶链反应( RT-PCR )分析显示在主机蛹的胖身体的 MSPH 和 BBP 的抄本层次响应寄生状态是可诱导的，他们的变化与在寄生状态以后的这些基因的翻译变化一致，当 Eno 和 IDGF 的抄本层次没被寄生状态影响时。这研究将贡献与天生的有免疫力的回答， detoxification，和精力新陈代谢联系的 parasitoidinduced 主人改变的分子的底的更好的理解。

英文摘要：

Parasitism by the endoparasitoid wasp Pteromalus puparum causes alterations in the plasma proteins of Pieris rapae. Analysis of plasma proteins using a proteomic approach showed that seven proteins were differentially expressed in the host pupae after 24-h parasitism. They were masquerade-like serine proteinase homolog （MSPH）, enolase （Eno）, bilin-binding protein （BBP）, imaginal disc growth factor （IDGF）, ornithine decarboxylase （ODC）, cellular retinoic acid binding protein （CRABP）, and one unknown function protein. The full length cDNA sequences of MSPH, Eno, and BBP were successfully cloned using rapid amplification of cDNA ends-polymerase chain reaction （RACE- PCR）. Reverse transcriptase-polymerase chain reaction （RT-PCR） analysis indicated that the transcript levels of MSPH and BBP in the fat bodies of host pupae were inducible in response to the parasitism and their variations were consistent with translational changes of these genes after parasitism, while the transcript levels of Eno and IDGF were not affected by parasitism. This study will contribute to the better understanding of the molecular bases of parasitoidinduced host alterations associated with innate immune responses, detoxification, and energy metabolism.