中文摘要：

目的：观察不同培养环境对小鼠视网膜神经细胞的影响。 方法：分别培养新生小鼠的视网膜神经细胞和色素上皮细胞,并纯化培养视网膜神经节细胞,将纯化和未纯化的视网膜神经细胞分别与视网膜色素上皮细胞接触和分层共培养,相差显微镜观察视网膜神经细胞在不同培养模式中的表现。 结果：混合培养的视网膜神经细胞较纯化培养的神经节细胞生存时间长,突触联系多。与视网膜色素上皮细胞共培养时,神经细胞的存活时间进一步延长,突触联系增多,但是神经细胞在与视网膜色素上皮细胞分层和接触共培养的两种模式中,显示出了不同的细胞形态。 结论：视网膜神经胶质细胞和视网膜色素上皮细胞不但对神经节细胞具有滋养作用,细胞间的立体结构关系可能还影响着细胞的成熟分化。

英文摘要：

AIM： To explore the effect of different culture environments on routine retinal neural cells. METHODS： Neonatal murine retinal neural cells, purified retinal ganglion cells and pigment epithelial cells were cultured. Purified and unpurified retinal ganglion cells were cultured with RPE in contacting or uncontacting modes. Appearance of neural cells was observed under phase-contrast microscope. RESULTS： Compared with purified ganglion cells, mixed retinal neural cells survived longer and had more synapse. Being cultured with RPE further, both mixed retinal neural cells and purified ganglion cells had longer survive time and more synapse. However, neural cells in contacting mode showed different appearance. CONCLUSION; In addition to providing nourishment to retinal ganglion cells, retinal glial cells and pigment epithelial cells also affect the differentiation and maturation of retinal neural cells by their stereo-structures.