中文摘要：

目的：观察富血小板血浆凝胶（PRG）析出液对骨髓间充质干细胞（BMSCs）迁移的影响。方法：全骨髓贴壁法体外培养犬BMSCs,二次离心法分离提取富血小板血浆并制备其凝胶析出液;将第3代BMSCs接种于Transwell小室,分别用含PRG析出液体积分数为0%（对照组）、1%、5%、10%、20%、50%的DMEM进行培养,观察5%PRG析出液作用不同时间（12、24、36 h）对BMSCs迁移活性的影响。结果：当培养液中含PRG析出液的浓度为1%、5%、10%、20%时,能明显促进BMSCs的迁移（P﹤0.05）,而当PRG析出液浓度为50%时,则抑制BMSCs的迁移（P﹤0.05）;5%PRG析出液促迁移作用最强,此后随PRG析出液浓度升高,促迁移作用越来越低,各浓度组间两两相比差异均有统计学意义（P〈0.05）;5%PRG析出液组作用于BMSCs 12、24、36 h均能明显促进BMSCs迁移（P〈0.05）,12 h组低于24 h和36 h组（P﹤0.05）,24 h组与36 h组相比P﹥0.05。结论：含1%、5%、10%、20%PRG析出液的培养基均可明显促进BMSCs的迁移,在一定浓度范围内呈时间依赖性。

英文摘要：

AIM： To investigate the effect of Platelet-rich plasma gel （PRG） precipitation liquid （PL） on the migration of bone marrow stem cells （BMSCs） in vitro. METHODS ： Canine BMSCs was obtained by adherence separation method. PRGPL was prepared by duplicatese centrifugation and diluted with DMEM to a volume fraction of 1%, 5%, 10%, 20% and 50% solution, BMSCs were cultured with PRGPL at the various concentrations for 24 h at 0（control） and 5% for 12 h, 24 h and 36 h respectively. The migration of BMSCs was examined by migration assay with transwell migration model. RESULTS： PRGPL at 1% ,5%, 10% and 20% promoted the migration of BMSCs （ P 〈 0.05 ） ; while at the concentration of 50% restrained the migration（ P 〈 0.05 ）. PRGPL at 5% showed the maxi- mum effect and time-dependeant migration increase （ between 12 h and 24 h culture, P 〈 0.05 ）, between 24 h and 36 h culture, P 〉 0.05）. CONCLUSION： The platelet-rich plasma gel precipitation liquid at concentrations of 1%, 5% ,10% ,20% can promote the migration of BMSCs, this migration effect is in a time-dependent manner.