中文摘要：

目的：探讨bFGF＋IGF1、TGFβ1＋BMP4、bFGF＋IGF1＋TGFβ1＋BMP4对体外培养的大鼠牙乳头细胞（rDPCs）增殖、分化的影响并筛选出最佳的组合因子。方法：分离培养、鉴定rDPCs,应用四唑盐（MTT）比色法和酶动力学法分别检测不同组合因子刺激后的第1/4/7/10/14天rDPCs的增殖活性、细胞内总蛋白合成量、碱性磷酸酶（ALP）的分泌水平,应用罗式诊断分析法和放射免疫分析法检测细胞上清液内钙、磷浓度和骨钙素含量。结果：组①bFGF（10ng/mL）＋IGF1（100ng/mL）在P4d能最显著地促进rDPCs的增殖、分化与矿化,并在P10d能显著地升高细胞外液的钙离子、磷离子浓度;组②TGFβ1（5ng/mL）＋BMP4（10ng/mL）在P4d能最大程度地促进rDPCs的分化、矿化并显著促进细胞外液骨钙素的分泌合成,且在P7d能最显著地促进rDPCs内总蛋白的合成（P〈0.05）。结论：bFGF＋IGF1、TGFβ1＋BMP4两组因子均可促进rDPCs的增殖活性和分化潜能,为体外牙齿再生提供实验依据。

英文摘要：

Objective：To investigate the effects of bFGF＋IGF1,TGFβ1＋BMP4,bFGF＋IGF1＋TGFβ1＋BMP4 on proliferation and differentiation of rat dental papilla cells （rDPCs） cultured in vitro and screen optimal combined factor.Methods：RDPCs were isolated,cultured,and identified in vitro.MTT chromatometry and enzyme dynamics assays were used to determine rDPCs proliferation activity,total protein synthesis in rDPCs and secretion level of alkaline phosphatase （ALP） respectively at 1/4/7/10/14d after stimulated by different combined factors.Roche diagnosis analytical method and radio immunoassay were performed to detect the concentration of calcium and phosphor,and osteocalcin （OCN） content in cell supernate.Results：bFGF（10ng/mL）＋IGF1（100ng/mL）could promote proliferation,differentiation and mineralization of rDPCs markedly at P4d,and advance concentration of calcium and phosphor within extracellular fluid notably at P10d.TGFβ1（5 ng/mL）＋BMP4（10ng/mL）could enhance differentiation and mineralization of rDPCs to maximal extent at P4d,and promote the secretion and synthesis of OCN within extracellular fluid significantly.Moreover,TGFβ1 （5ng/mL）＋ BMP4（10 ng /mL）could facilitate the total protein synthesis of rDPCs notably at P7d（P 0.05）.Conclusions：bFGF＋IGF1 and TGFβ1＋BMP4 could promote rDPCs proliferation activity and differentiation potentiality,which provide experimental bases for tooth regeneration in vitro.