中文摘要：

目的 观察三叉神经节神经元胞体中钠通道蛋白Nav1．8、Nav1．9mRNA在大鼠拔牙后的表达及作用，探讨拔牙术后外周组织疼痛的机制。方法Wister成年鼠30只，分为5组，每组6只，拔除大鼠右下磨牙，取同侧与对侧自体对照。采用体外快速构建cRNA标准曲线实时（realtime，RT）反转录聚合酶链反应（PCR）法，分别检测拔牙后30min、2h、1d、3d、6d时间点Nav1．8、Nav1．9mRNA的表达。结果Nav1．8、Nay1．9mRNA在拔牙后30min无明显变化，2h开始缓慢上调，至第3天时表达明显上调。在第3天Nav1．8、Nav1．9mRNA与对照组相比，分别上调27．0％、24．5％（P〈0．05），第6天表达开始下降。结论拔牙后疼痛产生与钠通道蛋白Nav1．8、Nav1．9mRNA表达上调有关，提示钠通道蛋白参与拔牙后外周组织疼痛的调节。

英文摘要：

Objective To observe the expression and function of extraction. Methods Real-time reverse transcription PCR paralleled with vitro-established cRNA standard curves was applied to measure the expression of Nav1. 8, Nav1. 9 at 30 min, 2 h, 1 d, 3 d and 6 d respectively after extraction of rat right mandibular molars. The right mandibular molars were used as control. Results Both Nav1. 8 and Nav1. 9 mRNA in right trigeminal ganglion showed little change after 30 min, and increased slowly after 2 h. Nav1. 8, Nay1.9 mRNA expressions increased by 27% and 24. 5% respectively compared to the left trigeminal ganglion after 3 d, reaching the highest level （ P 〈 0. 05 ） , and then the expressions began decreasing from 6 d. Conclusions The pain caused by molar extraction is related to the up-regulation of expressions of sodium channels protein Navl. 8 and Nav1. 9 mRNA, indicating the participation of sodium channels in regulations of peripheral tissue pain after molar extraction.