中文摘要：

用一种新的多台阶梯度洗脱的反相高效液相色谱（RPLC）法，首次建立了秦岭山区产4种产地及亚属均不相同的淫羊藿属药材的由9种成分组成的色谱指纹图谱．并以淫羊藿甙为内标作为相对标准，对其质量进行了评估．然而，因其产地和亚属的不同，指纹图谱又各有特征，发现产地不同的两种淫羊藿亚属，箭叶淫羊藿的RPLC指纹图谱由12个色谱峰组成．还提出用相对含量比较法对各种类淫羊藿中9种成分的含量进行了质量评价．本工作所建立的淫羊藿属药材的RPLC指纹图谱法从试样提取的精密度、稳定性、图谱重复性等方面均符合《中药注射剂指纹图谱研究的技术要求（暂行）》中的有关规定．该方法具有重现性好，特征性强，方法简便、快速等特点，有可能成为淫羊蓉药材种属判断、质量评估的标准，也会对未来开发淫羊藿中其他有效成分提供科学的依据．

英文摘要：

With a new multi-step gradient elution, a reversed phase high performance liquid chromatographic （RPLC） fingerprints chromatogram of the four kinds of Epimedium L. crude drugs producing in Qirding Mountainous area of China was firstly established. The common RPLC fingerprints of the Epimedium L. crude drugs contain nine components including icariin. The icatiin was selected as the internal standard and in terms of the relative amount of the nine components in the RPLC fingerprints of the four kinds of Epimedium L., the quality of the crude drugs was also evaluated. However, because of different producing areas and subgenus, each of the four RPLC fingerprints has specific character themselves. In addition, it is also found that the fingerprints of different producing areas of the Epimedium sagittatumm Maxim consist of twelve components. The RSD for the precision, stability and repeatability of all measurement are less than 3%. This method is simple, rapid, stable and has a good reproducibility. It may be used to identify the kind and to evaluate the quality of Epimedium L. crude drugs, and also to provide the scientific evidence for exploring some of available component of this drugs in the future.