中文摘要：

目的 观察黄连解毒汤水提物有效部位（总生物碱、总黄酮、总环烯醚萜）对脑缺血大鼠半暗带神经核抗原（NeuN）、半胱氨酸天冬氨酸蛋白酶3（Caspase-3）与多聚ADP核糖多聚酶（PARP）表达的影响,探讨其对神经元的保护作用机制.方法 采用大鼠永久性大脑中动脉阻塞模型.雄性SD大鼠随机分成假手术组、模型组、黄连解毒汤水提物（800 mg/kg）组、总生物碱组（44 mg/kg）、总黄酮组（50 mg/kg）、总环烯醚萜组（80 mg/kg）,连续灌胃给药7d,每天给药1次.免疫荧光染色结合图像分析技术检测NeuN、Caspase-3及PARP的表达.结果 模型大鼠脑缺血半暗带皮层NeuN阳性细胞数目减少,神经元NeuN表达降低,Caspase-3、PARP阳性表达明显增强,与假手术组比较差异显著（P＜0.01）；黄连解毒汤水提取物、总生物碱、总黄酮、总环烯醚萜组大鼠缺血半暗带皮层NeuN阳性细胞增多,神经元NeuN表达增强,Caspase-3、PARP表达下调,与模型组比较均差异显著（P＜0.05）.结论 黄连解毒汤水提取物、总生物碱、总黄酮、总环烯醚萜可通过抑制Caspase-3活性、降低PARP的过度表达,保护缺血半暗带神经元.

英文摘要：

Objective To observe the influences of Huanglian Jiedu tang＇ s active fractions, which consists of total alkaloids, total flavonoids and total iridoids, on the expressions of penumbra neuronal nuclei （NeuN）, cysteine aspartic acid proteinase 3 （Caspase-3） and poly-ADP-ribose polymerase （PARP） in rats after cerebral ischemia, and to discuss the mechanism of protective effects of these fractions on neurons. Methods The rat model of permanent middle cerebral artery occlusion （pMCAO） was chosen. Male SD rats were randomly divided into sham-operation group, model group, aqueous extract group （800 mg/kg）, total alkaloids group （44 mg/kg）, total flavonoids group （50 mg/kg） andtotal iridoids group （80 mg/kg）. All groups were orally given corresponding medieinals once a day for 7 d. The expressions of NeuN, Caspase-3 and PARP were detected by using immunofluorescence staining combined image analysis technique. Results In model group, the number of NeuN positive ceils decreased in ischemic penumbra cortex decreased, expression of NeuN decreased, and expressions of Caspase-3 and PARP increased significantly compared with sham-operation group （P 〈 0. 01 ）. In aqueous extract group, total alkaloids group, total flavonoids group and total iridoids group, the number of NeuN positive cells increased in ischemic penumbra cortex, expression of NeuN increased, and expressions of Caspase-3 and PARP decreased compared with model group （ P 〈 0.05 ）. Conclusion The aqueous extract, total alkaloids, total flavonoids and total iridoids in Huanglian Jiedu Tang have protective effect on neurons in ischemic penumbra cortex through depressing the activity of Caspase-3 and decreasing the over-expression of PARP.