中文摘要：

Kinesins 在有编码 Kinesin-14B 亚科的一个成员的多样的 functions.A cDNA 的许多真核细胞的房间是普通的用 3'-RACE 技术和 namedAtKP1 被获得(为 Arabidopsis kinesin 蛋白质 1 ) 。这 cDNA 有 3.3 kbencoding 的一个最大的开的读物框架 1087 的多肽一一。蛋白质领域分析证明 AtKP1 分别地在中央、氨基终端的区域包含马达领域和 calponin 相同领域。有 202aa 残余的 Thecar-boxyl-terminal 区域从另外的已知的 kinesins 是多样的。北污点分析比在 AtKP1 倡导者的成熟 plants.2808 bp 区域被克隆并且熔化到 GUS 证明 AtKP1 广泛地在幼苗在高水平被表示。AtKP1promoter 区域驾驶的 GUS 表示证明 AtKP1 主要在年轻机关和年轻的叶毛状体的 vasculature 被表示，显示 AtKP1 可以参予区别或发展 ofArabidopsis thaliana 脉管的捆和毛状体。包含通常认为的马达领域的 Atruncated AtKP1 蛋白质在 E 被表示。关口 i 并且净化亲密关系。在 vitro，描述显示多肽有核苷酸依赖者微导管绑定能力刺激 andmicrotubule 的 ATPase 活动。

英文摘要：

Kinesins are common in a variety of eukaryotic cells with diverse functions. A cDNA encoding a member of the Kinesin-14B subfamily is obtained using 3＇-RACE technology and named AtKP1 （for Arabidopsis kinesin protein 1）. This cDNA has a maximum open reading frame of 3.3 kb encoding a polypeptide of 1087 aa. Protein domain analysis shows that AtKP1 contains the motor domain and the calponin homology domain in the central and amino-terminal regions, respectively. The carboxyl-terminal region with 202 aa residues is diverse from other known kinesins. Northern blot analysis shows that AtKP1 is widely expressed at a higher level in seedlings than in mature plants. 2808 bp of the AtKP1 promoter region is cloned and fused to GUS. GUS expression driven by the AtKP1 promoter region shows that AtKP1 is mainly expressed in vasculature of young organs and young leaf trichomes, indicating that AtKP1 may participate in the differentiation or development of Arabidopsis thaliana vascular bundles and trichomes. A truncated AtKP1 protein containing the putative motor domain is expressed in E. coil and affinity-purified. In vitro characterizations indicate that the polypeptide has nucleotide-dependent microtubule-binding ability and microtubule-stimulated ATPase activity.