中文摘要：

应用衔接头PCR技术，以蓝猪耳（Torenia foumien）全基因组DNA分别经DraI、EeoRⅤ、PvuⅡ和SmaⅠ消化后与衔接头连接产物为模板，用衔接头引物和TfPLC1基因的特异引物经过多轮的巢式PCR，先后克隆到2个798和813bp的Tf-PLC1基因上游序列；经测序、blast比较分析和拼接得到一个蓝猪耳TfPLC1基因的启动子序列，共1432bp。序列分析表明它含有类似于TATAbox和CAATbox的元件，在其远端上游区域还有多个AT富含区，而且还含有多个胚乳特异表达启动子的元件。将该启动子全序列和5’端缺失的700bp序列与PBI121分别构建了植物表达载体PPP1326和PPP700，用于转化蓝猪耳，以验证该启动子的功能。

英文摘要：

Torenia fournieri genomic DNA was digested by Dra Ⅰ, EcoR V, Pvu Ⅱ and SrnaⅠ respectively. A special adaptor was ligated to the ends of the digested DNA fragments as a template for adaptor PCR. With the adaptor and TfPLC1 gene-specific primers, bands of 798 and 813 bp upstream of TfPLC1 were obtained successively. After sequenced, blast and combined, a TfPLC1 promoter of 1432 bp was gained. TATA box and CAAT box-like elements were found in the sequence, and AT domains were rich in the far upstream of the promoter. And also some endosperm-specific elements were found in the sequence. PBI121 and the promoter were double digested to construct plant expression vectors PPPI326 and PPP700 by 5＇deletion. Then the vectors were transformed to Torenia fournieri to validate the function of the promoter.