中文摘要：

以拟南芥（Arabidopsis thaliana）为材料，运用RTPCR技术扩增得到了富含亮氨酸的类受体蛋白激酶（LRRRLKs）亚家族基因RLK6，构建了RLK6与绿色荧光蛋白基因（GFP）融合表达载体并转化拟南芥，用激光共聚焦扫描显微镜观察转基因植物细胞表明：RLK6蛋白定位于细胞膜上；将RLK6-GFP在原生质体中进行瞬时表达，进一步证实了RLK6蛋白定位于细胞膜上。构建了RLK6启动子（2063bp）融合GUS报告基因的载体并转化拟南芥，对转基因植株进行组织化学染色分析表明：RLK6在拟南芥的幼苗、根、花、角果等组织中都有表达，花中表达量较高，尤其是在雄蕊中特异高表达，而在茎、莲座叶和干种子中几乎没有表达。RTPCR分析结果与GUS组织化学染色的结果一致。研究推测，RLK6可能在花器官生长发育或相关生理过程的信号转导中发挥作用。

英文摘要：

The full length coding sequence without the TAA stop codon of LRR-RLKs gene （RLK6） of Ar- abidopsis thaliana was cloned by RT-PCR and fused with green fluorescent protein （GFP） gene to construct a plant expression vector. The stable Arabiclopsis transformants were observed using confocal laser scanning microscope. The result indicated that RLK6-GFP fusion protein was localized in the plasma mem- branes. Consistent location was obtained by the experiment of RLK6-GFP transient expression in living Arabidopsis protoplasts. To visualize the temporal and spatial expression patterns RLK6 in plant tissue, a 2 063 bp promoter fragment of RLK6 was fused with the GUS gene to generate transgenic plants. GUS ac- tivity was detected in seedlings, roots and flowers, especially in anther,but hardly detected in stems, rosette leaves and dry seeds. Consistent result was obtained by RT-PCR analysis. Overall, these data implied that RLK6 might involve in the signaling pathway of flower organs development or related processes.