中文摘要：

目的：探讨黑灵芝多糖（polysaccharide from Ganoderma atrum，PSG-1）对S-180荷瘤小鼠腹腔巨噬细胞环磷酸腺苷（cyclic adenosine monophosphate，cAMP）/细胞蛋白激酶A（protein kinase A，PKA）、三磷酸肌醇（inositol triphosphate，IP3）/Ca2＋及甘油二酯（diacylglycerol，DAG）/蛋白激酶C（protein kinase C，PKC）信号通路的影响。方法：将S-180细胞接种于BALB/c小鼠体内建立荷瘤小鼠模型，成模后收集腹腔巨噬细胞进行体外培养，用不同浓度的PSG-1干预；ELISA法分别检测巨噬细胞培养上清液中的IP3、DAG和AMP含量；流式细胞仪测定细胞内Ca2＋含量；Western blotting测定细胞PKA及PKC蛋白表达。结果：PSG-1在20～160 μg/mL质量浓度范围能促进S-180荷瘤小鼠腹腔巨噬细胞产生cAMP、IP3和DAG，同时能增加细胞内Ca2＋含量，并显著增强PKA及PKC蛋白表达量。结论：PSG-1能有效激活S-180荷瘤小鼠腹腔巨噬细胞cAMP/PKA、IP3/Ca2＋及DAG/PKC信号通路。由此推测，PSG-1可能通过cAMP/PKA、IP3/Ca2＋及DAG/PKC信号通路促进S-180荷瘤小鼠腹腔巨噬细胞抗肿瘤作用。

英文摘要：

Objective： To explore the effects of a polysaccharide from Ganoderma atrum （PSG-1） on cyclic adenosine monophosphate （cAMP）/protein kinase A （PKA）, inositol triphosphate （IP3）/Ca2＋ and diacylglycerol （DAG）/protein kinase C （PKC） signaling pathways in peritoneal macrophages of S-180 tumor-bearing mice. Methods： A tumor-bearing mouse model was established by inoculating mouse sarcoma S-180 cells into BALB/c mice; peritoneal macrophages were collected from the S-180 tumor-bearing mice, then cultured in vitro and treated with PSG-1 at various concentrations. The IP3, DAG and cAMP levels in cell culture supernatant were measured by ELISA. The intracellular Ca2＋ content was assayed by a flow cytometric method. The PKA and PKC protein expression in macrophages was determined by Western blotting. Results： PSG-1 in the concentration range of 20-160 μg/mL stimulated the production of IP3, DAG and cAMP in the macrophages from S-180 tumor-bearing mice, increased the intracellular Ca2＋ content, and increased the protein expression of PKA and PKC. Conclusion： PSG-1 can exert anti-tumor activity in peritoneal macrophages of S-180 tumor-bearing mice through the activation of cAMP/PKA, IP3/Ca2＋ and DAG/PKC signaling pathways.