中文摘要：

目的：研究人体组织Malassez上皮剩余对炎症组织来源的牙周膜干细胞成骨能力的影响。方法：分别从牙周炎患者及健康者的牙周组织中获得牙周膜细胞，并分别采用低密度法筛选纯化出炎症牙周膜干细胞；差速消化法获得Malassez上皮剩余。用流式细胞术对炎症牙周膜干细胞进行表型鉴定，相差显微镜观察Malassez上皮剩余形态，用transwell 小室构建Malassez上皮剩余与炎症牙周膜干细胞的共培养体系。然后取小室的下层炎症牙周膜干细胞，用RT－PCR技术检测ALP和Runx－2 mRNA表达水平；ALP染色及茜素红染色观察其成骨分化能力的变化。结果：成功获得了炎症牙周膜干细胞和Malassez上皮剩余，并顺利构建共培养体系；经成骨诱导后，与Malassez上皮剩余共培养的炎症牙周膜干细胞与单独培养组相比，成骨相关基因ALP、Runx－2 mRNA水平均明显上调（P＜0．05），ALP染色加深，形成的矿化结节也明显增多。结论：将炎症牙周膜干细胞与Malassez上皮剩余共培养后可使其成骨分化能力增强。

英文摘要：

AIM：To study the effects of epithelial cell rests of Malassez （ERM）on the osteogenesis capaci-ty of periodontal ligament stem cells derived from periodontitis tissue （PPDLSCs）.METHODS：PPDLSCs were cul-tured by low density inoculation and identified by flow cytomety.Human ERM was obtained by differential digestion and observed under phase-contrast microscope.ERMand PPDLSCs were co-cultured with 0.4 μm transwell.ALP and Runx-2 mRNA expression was examined by real-time PCR,the mineralization of the culture was evaluated by ALP staining and Alizarin Red staining.RESULTS：PPDLSCs co-cultured with ERM expressed higher mRNA levels of ALP and Runx-2.Furthermore,co-cultured PPDLSCs had stronger ALP activity and produced more mineralized nod-ules.CONCLUSION：The osteogenesis capacity of PPDLSCs can be enhanced by co-culture with ERM.