中文摘要：

目的：探讨体外模拟正畸牙移动过程中机械压力对人牙周膜干细胞（HPDLSC）凋亡相关蛋白表达的影响。方法：将体外培养的HPDLSC分别在自行研发的加压力装置中进行100、200 Kpa加压1、6和12 h后,检测和比较其凋亡相关蛋白caspase-3和caspase-8的表达;流式细胞仪检测其凋亡状态。结果：100 Kpa加压1 h的HPDLSCs中caspase-3和caspase-8的蛋白表达及其凋亡水平均显著高于对照组（P〈0.05）,而加力12 h的HPDLSCs中caspase-3和caspase-8的蛋白表达和凋亡水平与对照组比较无统计学差异（P〉0.05）。200 Kpa加力1 h的HPDLSCs中caspase-3和caspase-8的蛋白表达和凋亡水平均显著低于对照组（P〈0.05）,而加力12 h的HPDLSCs中caspase-3和caspase-8的蛋白表达和凋亡水平与对照组比较无统计学差异（P〉0.05）。结论：在体外模拟正畸牙移动过程中,不同强度静态压力作用不同时间对人牙周膜干细胞的凋亡作用不同,100 Kpa静态加力加压1 h可更有效地促进人牙周膜干细胞的凋亡,而200 Kpa静态加力加压1 h可抑制人牙周膜干细胞的凋亡。

英文摘要：

Objective： To investigate the effect of static pressure on the expression of apoptosis related protein in HPDLSCs. Methods： HPDLSCs cultured in vitro were incubated under continuous static pressure of 100 Kpa and 200 Kpa for 1 h, 6 h and 12 h. The expression of apoptosis related protein caspase-3 and caspase-8 in HPDLSCs were tested by Western Blot. The apoptosis level of HPDLSCs was determined by Flow cytometry. Results： Under the static pressure of 100 Kpa for 1 h, both the expressions of the apoptosis related protein caspase-3 and caspase-8 and the level of apoptosis increased more significantly than that of the controls （P〈0.05）, but no statistical significance was found when the static pressure continued for 12 h （P〉0.05）. Under the static pressure of 200 Kpa for 1 h, both the expressions of the apoptosis related protein caspase-3 and caspase-8 and the level of apoptosis were lower than that of the controls （P〈0.05）, but no statistical significance was found when the static pressure continued for 12 h （P〉0.05）. Conclusions： Continuous static pressure of 100 kpa for 1 h promotes apoptosis, while continuous static pressure of 200 kpa for 1 h inhibits apoptosis.