中文摘要：

为了验证天山雪莲△9硬脂酰脱饱和酶基因sikSAD信号肽的功能,本研究利用PCR法从已构建的天山雪莲cDNA文库中克隆了sikSAD的96bp信号肽序列,将其与报告基因GFP连接,成功构建了融合基因植物表达载体pCMBIA2301-sp-GFP。通过农杆菌介导的叶盘法转化烟草NC89,获得转基因植株。通过激光共聚焦显微镜观察GFP在转基因烟草叶片中的激发荧光,确定天山雪莲△9硬脂酰脱饱和酶sikSAD定位于天山雪莲的叶绿体中。

英文摘要：

To authenticate the function of sikSAD ＇s signal peptide,the 96 bp signal peptide sequence by using PCR from cDNA library of Sasussured involucrate Kar.et Kir which had been constructed was cloned,then ligated with report gene GFP,constructed a plant expression vector pCMBIA2301-sp-GFP.Transformed them into tobacco NC89 by Agrobacterium-mediated transformation method,and got the transgenic plant.Subcellular localization of sikSAD was examined by laser confocal microscope by using the approaches of GFP-tagging.