中文摘要：

【目的】构建吕氏泰勒虫裂殖子cDNA表达文库，并从中筛选抗原候选基因。[方法]从吕氏泰勒虫裂殖子直接提取和纯化mRNA，采用o1igo（dT）引物合成双链cDNA，并在其两端加如EcoRI／Hind III定向接头。将所产生的cDNA分子定向克隆到具有EcoRI／Hind III粘性末端的入SCREEN载体的两臂之间。用PhageMaker extract对连接产物进行体外包装以形成完整的噬菌体，并用之转染大肠杆菌ER1647，从而构建成吕氏泰勒虫的cDNA表达文库。用吕氏泰勒虫阳性血清和兔抗绵羊IgG-AP筛选得到阳性克隆，经测序和Blast软件分析并获得新基因。【结果】成功构建吕氏泰勒虫裂殖子cDNA表达文库，其初级库容量约为1．0×10^6PFU，扩增文库的滴度为8．2×10^8PFU·ml^-1，文库重组率为100％；通过免疫学筛选、测序和Blast软件分析，共获得30个新基因，其中15个基因已登录GenBank／NCBI。[结论]为研究泰勒虫疫苗、新型医药和诊断抗原，以及发展可持续性防制羊泰勒虫病提供基本材料。

英文摘要：

[objective] A cDNA expression library of the merozoites of Theileria luwenshuni was successfully constructed and antigenic genes were obtained by immunoscreening from it. [Method] Purified mRNA was isolated from the merozoites of Theileria luwenshuni. A library of oligo （dT） -primed cDNA with added directional EcoR I IHindlII linkers was constructed and ligated to the EcoR I/Hind III arms of the screen vector. The recombinant phage DNA was packaged by using PhageMaker packaging extracts, resulting in a primary cDNA library. The positive clones were immunoscreened with the positive sera against Theileria luwenshuni and the rabbit anti-sheep IgG-AP from the library. The new genes were obtained by Blast anlysis and sequencing. [Result] A cDNA expression library of the merozoites of Theileria luwenshuni was successfully constructed, with a size of 1.0 ×10^6 PFU, amplified cDNA library with a phage titer of 8.2 ×10^8 PFU·ml^-1 , and a recombinant rate of 100%. Thirty new genes were obtained by analysis of immunoscreening, sequencing and Blast, and 15 of them have been submitted in GenBank/NCBI. [ Conclusion] The experiment has established an important material bases for the development of Theileria vaccine, new pattern medicine, diagnostic antigen and so on.