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中文摘要：
基于非洲猪瘟病毒（ASFV）VP72基因设计引物,建立一种检测非洲猪瘟病毒的PCR方法。应用本研究所建立的方法与OIE参考的方法进行比较,并对参考实验室提供的非洲猪瘟病毒17个分离株的基因组以及本实验室收集的野外样品进行检测。结果显示：本研究设计的引物具有良好的特异性,与猪的其他病原没有交叉反应;其敏感性与OIE参考的方法相当;所建立的PCR方法能够成功扩增非洲猪瘟病毒17个分离株的基因组,野外样品检测均为阴性。根据上述研究结果,本研究所建立的方法具有很好的应用性,能够用于非洲猪瘟疫病的诊断以及防控。
英文摘要：
A polymerase chain reaction（PCR） was developed for detection of African swine fever virus（ASFV） based on the VP72 gene.This method was compared with the PCR method which was recommended for diagnosis of African swine fever by OIE.Seventeen genomic DNAs of ASFV isolates from ASF reference laboratory and some field samples collected from China were detected by PCR,respectively.The results revealed that it had a good specificity,no cross-reaction was observed with other pig pathogens.The sensitivity was equivalent to the PCR which was recommended by OIE.Seventeen genomic DNAs of ASFV isolates could be detected successfully,but all of the field samples were negative.This method has a good applicability and could be used in diagnosis,prevention and control of African swine fever.
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