Discrete Fourier Transform (DFT) based multiple-input multiple-output Orthogonal Frequency Division Multiplexing (MIMO-OFDM) is the focus of wireless communication, which uses cyclic prefixes to reduce the ISI. To improve the spectrum efficiency and system performance, Complex Wavelet Packet Transform (CWPT) based OFDM is used to realize the MIMO-OFDM system. For the good property of complex wavelet packet function, the CWPT based MIMO-OFDM system is better than the DFT based MIMO-OFDM system, but the CWPT based MIMO-OFDM scheme has some additional complexity, and simulation results show that the new system can improve the system performance.