目的为建立封闭群实验猕猴,对皖南山区猕猴种群的遗传背景进行了初步调查。方法活体测量了18只猕猴的形态特征变量,并使用微卫星标记对33只猕猴进行了遗传多样性分析。结果体重变量具有显著的性别差异,躯干变量具有显著的年龄差异。同时从12个微卫星标记中筛选出9个具多态性标记,在猕猴群中平均检测到8个等位基因,群体杂合度H=0.825±0.106,多态信息含量PIC=0.788±0.121。结论皖南山区猕猴种群的形态特征体现了福建亚种的特性,在微卫星水平上表现出较高的遗传多样性,适合进行引种并建立猕猴的封闭群。
Objective In order to establish the closed colony, the heredity background of the rhesus monkeys from Anhui stock were investigated. Method Several morphological variables of 18 individual monkeys from Anhui Wannan Mountains were measured, and the genetic polymorphism of 33 monkeys was analyzed by microsatellite loci. Result The results of t-test showed that there were evident differences in weight between male and female and that in trunk length between adult and subadult. At the same time, nine polymorphic microsatellite loci were selected from twelve loci. The mean number of alleles of Anhui rhesus monkey stock detected was 8. The heterozygosity value was 0.825±0.106, and the polymorphism information content value was 0.788±0.121. Conclusion Morphological traits of Anhui rhesus monkey stock embodied the feature ofM.mulatta littoralis. And genetic diversity of the group is high on the microsatellite level. So we can screen out the original seed from Anhui rhesus monkey stock and establish the closed colony.