中文摘要：

为检测在野外通过非损伤取样法采集到的短尾猴样品是否受到其它常见哺乳动物及人源的污染，收集包括短尾猴（Macaca thibetana）、人以及7种常见哺乳动物的毛发、血液、粪便、精液或肌肉组织样品，提取并纯化基因组DNA，利用14对微卫星引物扩增不同物种的STR位点，以确定其在短尾猴样品检测中的应用价值。扩增结果表明，TH01等13对引物在人和短尾猴中均能扩增且扩增产物长度未见明显差异，F13A01产物长度在人和短尾猴之间相差240bp碱基，只有FGA位点在常见动物猪、羊、牛、狗、兔、小白鼠及豚鼠都未见扩增产物；因此，FGA、F13A01座位较高的分辨能力，可以排除短尾猴样品是否受到人及常见哺乳动物的污染，通过特异性STR引物结合PCR方法，检测短尾猴野外非损伤取样获得的样品是否受到哺乳动物及人源DNA污染，此方法简单、稳定性高、可重复性好，在短尾猴的遗传多样性分析及亲缘关系鉴定中有较大的应价值。

英文摘要：

To detect whether Tibetan macaque samples were contaminated by human and other common animals biological samples. Hairs, blood, faeces, sperm and muscle tissues of Tibetan macaque, human and seven other kinds of common animal samples were collected for PCR. DNA was quantified after extraction and purification. 14 microsatellite primers were used in this study. The amplification products of Tibetan macaque FGA loci had one band of 200 bp which is almost the same as in human beings. No products were found in rabbit, pig, cattle, rat, cavy, sheep and dog. The TH01, CSF10PO）, F13A01 and all the other loci could be amplified in human and Tibetan macaque, but the difference was only found in F13A01 products. Those results suggest that the primers for human FGA, F13A01 loci can be used to distinguish Tibetan macaque from human beings and common animals. They are two useful and sensitive STR loci in Tibetan macaque＇s genetic diversity and parentage identification.