研究以采自吉林和江西的水稻土为供试土壤,采用恒温泥浆厌氧培养方法,探讨添加生物炭和不同有机碳源(葡萄糖、乙酸钠、丙酮酸钠和乳酸钠)条件下土壤泥浆中Fe(Ⅱ)浓度和pH值的变化,采用Logistic模型对Fe(Ⅲ)的还原特征进行了拟合分析.结果表明,添加生物炭可以促进2种水稻土中的Fe(Ⅲ)还原能力.添加生物炭条件下,不同外源有机碳对水稻土中Fe(Ⅲ)还原特征的影响存在差异,在吉林水稻土中,对Fe(Ⅲ)还原的调控能力显著大于江西水稻土.2种水稻土均能较好地利用乳酸盐、丙酮酸盐和葡萄糖还原Fe(Ⅲ),而添加乙酸盐后的Fe(Ⅲ)还原则表现出一定的滞后性.添加乳酸盐处理最大Fe(Ⅲ)还原速率高于其他有机碳源,且达到最大Fe(Ⅲ)还原速率的时间最短,表明乳酸盐是2种水稻土中铁还原过程的优势碳源.添加发酵性的有机碳源可显著影响泥浆培养过程中的pH值,有机碳源对江西水稻土pH的影响明显大于吉林水稻土,表明吉林水稻土中有机碳源对发酵过程产生的氢的利用能力优于江西水稻土.
In order to reveal the effect of biochar on carbon source utilization in Fe( Ⅲ ) reduction process of different paddy soils, the method of anaerobic slurry incubation under constant temperature was used on two paddy soils which were sampled from Jilin(YJ) and Jiangxi(NC) province, respectively, with addition of bioehar and different carbon sources (glucose, acetate, pyruvate and lactate). The Fe( Ⅱ) concentration and pH in the anaerobic slurry of two paddy soils were measured at regular intervals, and the data was fitted and analyzed by Logistic model. The results showed that the addition of bioehar eould promote Fe( Ⅲ) reduction process in two paddy soils. In the presence of biochar, effects of carbon sources on Fe (Ⅲ) reduction depended on paddy soils, which were more significant in YJ paddy soil than in NC paddy soil. Lactate, pyruvate and glucose can be well utilized by iron-reducing microorganism in both paddy soils, while acetate inhibited the reduction of Fe( Ⅲ) to some extent. Highest maximum Fe( Ⅲ ) reduction rate and shortest time to reach the maximum Fe( Ⅲ ) reduction rate were observed in lactate treatment, irrespective of paddy soils, which showed that lactate was the superior carbon source in Fe( Ⅲ ) reduction process of both paddy soils. The effects of fermentative carbon sources on soil pH were more significant in NC paddy soil than those in YJ paddy soil during anaerbic incubation, which indicated that H2 produced in fermentative process could be better utilized by microorganisms in YJ paddy soil than those in NC paddy soil.