采用发光二极管(light-emitting diode,LED)调制光质和光量,研究光谱能量分布对冬青试管苗生长的影响,以荧光灯为对照。结果表明:红光LED处理下试管苗的干样质量、生根率、根冠比和根长指标最高;而蓝光LED处理下则最低。绿光LED有利于叶片质量增加。单色红光LED导致试管苗徒长,根系活力和移栽成活率最低,最高值出现在复合光质LED组合中。与荧光灯相比,在能效、干样质量、碳水化合物含量以及SOD和CAT抗氧化酶活性指标上,复合光质LED具有明显优势,有利于培育壮苗、提高移栽成活率并降低能耗成本。
The effects of different light spectral energy distribution of light-emitting diode (LED) on growth of Ilex chinensis Sims plantlets in vitro were studied. Fluorescent light was used as the control. The results showed that, plantlets under red LED exhibited the highest dry mass, rooting rate, root/shoot rate and root length, but blue LED had the contrary effects. Green LED was suitable for leaf growth. However, monochromic red LED induced abnormal growth, poor root vigor and least survival rate. Compared with fluorescent light, complex of LED showed the obvious advantages on energy efficiency, dry mass, carbohydrate contents, SOD and CAT activities. The results indicated that application of complex of LED could improve antioxidant capacity and environment adaptable of Ilex chinensis Sims plantlets in vitro which got stronger and survival rate were higher than those under fluorescent.