中文摘要：

采用脉冲和连续波方式沉积等离子体聚烯丙胺薄膜改性聚酯（PET）材料表面，并进一步在等离子体聚烯丙胺薄膜表面固定肝素分子。利用衰减全反射红外光谱、X射线光电子能谱和接触角测试等离子体聚烯丙胺薄膜的元素成分、组成和表面能，采用对三氟甲基苯甲醛衍生法和甲苯胺蓝法分别检测了等离子体聚烯丙胺薄膜表面的伯胺基浓度和固定肝素分子的聚烯丙胺薄膜表面的肝素浓度。实验结果表明，脉冲等离子体聚合薄膜PPAa-P表面的伯胺基浓度为1.4％，而连续波等离子体聚合薄膜PPAa-C表面伯胺基浓度只有0．71％。等离子体聚烯丙胺薄膜改性的PET的表面能增加，其中PPAa-P改性的PET表面的表面能的极性分量增加较大。脉冲等离子体聚烯丙胺薄膜表面固定的肝素浓度为4.07μg／cm^2，为连续波等离子体聚烯丙胺薄膜表面固定肝素浓度2．23μg／cm^2的1．8倍。体外血小板粘附实验结果表明，表面肝素化的PET表面有较低数量的血小板粘附和激活，尤其是在固定肝素分子的脉冲等离子体聚烯丙胺薄膜改性的PET表面表现出更好的抗凝血性。

英文摘要：

The plasma polymerized allylamine films were fabricated to modify the surfaces of the polyethylene terephthalate （PET） by pulsed and continued plasma polymerization technique, then the molecules of heparin were immobilized on the polyallylamine films by chemical reaction. The surface properties of the film were de- termined by attenuated total reflection Fourier transform infrared spectroscopy （ATR-FTIR）, X-ray photoelec- tron spectroscopy （XPS）, contact angle measurement, and chemical analysis. The content of the primary amine group of pulsed plasma polymerized allylamine film （PPAa-P） and the concentration of heparin immobilized on the PPAa-P films are 1.40% and 4.07μg/cm^2 , which were higher than those on the continued plasma polymerized allylamine film （PPAa-C） and the heparin-immobilized PPAa-C film. Compared to PPAa-C film, PPAa-P film was better hydrophilicit}. The anticoagulative property of the PET, heparin-immobilized PPAa-P （H- PPAa-P） and heparin-immobilized PPAa-C （H-PPAa-C） films were evaluated via in vitro platelet adhesion. Compared with the untreated PET, platelet adhesion and activation on the surfaces H-PPAa-P and H-PPAa-C films were remarkably decreased. Especially, the surface of H-PPAa-P was found to be much better anticoagulative effectivity.