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甲硫醚降解菌JLM-8的分离鉴定与降解条件优化
  • ISSN号:0438-1157
  • 期刊名称:《化工学报》
  • 时间:0
  • 分类:Q939.9[生物学—微生物学]
  • 作者机构:[1]浙江工业大学生物与环境工程学院,浙江杭州310014
  • 相关基金:中国博士后科学基金面上项目(2014M560495);浙江省教育厅科研项目(Y201329224);教育部“长江学者和创新团队发展计划”(IRT13096)。
中文摘要:

从制药厂的活性污泥中分离到一株能以唯一碳源和硫源降解甲硫醚的菌株JLM-8,经过生理生化测试与16S rDNA系统发育树分析鉴定为嗜麦芽寡养单胞菌(Stenotrophomonasmaltophilia)。通过测定菌株的生长量、甲硫醚的降解率,利用响应面法优化最佳降解条件,并测定了该菌降解甲硫醚的动力学参数。结果表明:当接种量为25 mg·L-1时,通过响应面法优化的最佳降解条件为温度31.3℃、pH 7.5,初始甲硫醚浓度50 mg·L-1时最大预测降解率为98.2%,实验验证降解率为97.9%。菌株降解动力学参数最大比降解速率、半饱和系数、抑制系数分别为2.37 h-1、143.55 mg·L-1、51.35 mg·L-1,临界抑制浓度为78.46 mg·L-1。

英文摘要:

Dimethyl sulfide (DMS) is one of the most important volatile organic sulfur compounds. Due to its malodorous smell and the extremely low odor threshold, DMS has become a critical issue in wastewater collection and treatment systems. In this study, a novel strain JLM-8, with capability of degrading DMS as sole carbon and sulfur source, was isolated from the active sludge of a pharmaceutical plant. Based on the morphological, physiological and biochemical properties as well as its 16S rDNA gene sequence analysis, the strain JLM-8 was identified as a member of Stenotrophomonasmaltophilia. The strain could degrade approximately 100% of 50 mg·L-1 DMS within 8 hours at pH 7 and 30℃. Response surface methodology (RSM) analysis showed that the optimum conditions for degradation were at pH 7.5 and 31.3℃, using an inoculum amount of 25 mg·L-1. Under these conditions, approximately 97.9% of DMS was degraded. The kinetic parametersqmax,Ks, andKi were established to be 2.37 h-1, 143.55 mg·L-1, 51.35 mg·L-1, respectively. The critical inhibitor concentration was determined to be 78.46 mg·L-1. Research on the characterization of DMS degradable bacteria can provide a new theoretical and technical fundamental basis for bioremediation of DMS-contaminated environments.

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期刊信息
  • 《化工学报》
  • 中国科技核心期刊
  • 主管单位:中国科学技术协会
  • 主办单位:中国化工学会 化学工业出版社
  • 主编:李静海
  • 地址:北京市东城区青年湖南街13号
  • 邮编:100011
  • 邮箱:hgxb126@126.com
  • 电话:010-64519485
  • 国际标准刊号:ISSN:0438-1157
  • 国内统一刊号:ISSN:11-1946/TQ
  • 邮发代号:2-370
  • 获奖情况:
  • 中国科协优秀期刊二等奖,化工部科技进步二等奖,北京全优期刊奖,中国期刊方阵“双效”期刊,第三届中国出版政府奖
  • 国内外数据库收录:
  • 俄罗斯文摘杂志,美国化学文摘(网络版),荷兰文摘与引文数据库,美国工程索引,日本日本科学技术振兴机构数据库,中国中国科技核心期刊,中国北大核心期刊(2004版),中国北大核心期刊(2008版),中国北大核心期刊(2011版),中国北大核心期刊(2014版),中国北大核心期刊(2000版)
  • 被引量:35185