中文摘要：

目的 ：探讨脂多糖（lipopolysaccharide,LPS）诱导星形胶质细胞活化过程中细胞周期蛋白D3（cyclin D3）、周期蛋白依赖性激酶（cyclin-dependent kinase,CDK）11p58的表达、亚细胞定位及相互作用。方法：LPS刺激体外培养大鼠星形胶质细胞,采用Western Blot技术检测过程中cyclin D3及CDK11p58蛋白的表达情况;用免疫共沉淀技术分析LPS刺激前后cyclin D3及CDK11p58形成复合物的情况;用免疫荧光双标技术检测二者的亚细胞定位情况;将cyclin D3真核表达载体转染入星形胶质细胞,用核浆分离技术分析过表达cyclin D3对于CDK11p58亚细胞定位影响。结果：LPS呈浓度依赖性上调星形胶质细胞内cyclin D3及CDK11p58蛋白表达;cyclin D3与CDK11p58可形成复合物,LPS刺激增强二者相互作用;静息状态下星形胶质细胞内cyclin D3及CDK11p58均主要位于细胞核,但在细胞质内亦有不同程度表达,LPS刺激后二者均向细胞核聚集,且出现明显细胞核内共定位;过表达cyclin D3可促进CDK11p58向细胞核转运。结论：LPS可刺激大鼠星形胶质细胞内cyclin D3及CDK11p58蛋白表达;cyclin D3通过与CDK11p58相互作用,促进CDK11p58细胞核转运,从而参与星形胶质细胞活化过程。

英文摘要：

Objective： To study the expression,cellular localization and interaction of cyclin D3 and cyclin-dependent kinase（CDK） 11p58 during lipopolysaccharide（LPS）-induced rat astrocyte activation.Methods： During LPS-induced rat primary astrocyte activation,Western Blot was employed to detect the expression of cyclin D3,CDK11p58 and CDK11p110,co-immunoprecipitation technology was utilized to analyze the interaction between cyclin D3 and CDK11p58,immunofluorescence assay was used to detect their cellular localization.Finally,we transfected cyclin D3-overexpression plasmid into rat astrocytes,and evaluated the influence of cyclin D3 overexpression on the cellular distribution of CDK11p58.Results： LPS stimulated the protein expression of cyclin D3,CDK11p58,but not CDK11p110 in rat primary astrocytes in a dose-dependent manner.Cyclin D3 interacts with CDK11p110 to form the Cyclin D3-CDK11p58 complex,and LPS promotes their interaction.In resting astrocytes,cyclin D3 and CDK11p58 mostly localize in the nuclear but also appear in the cytoplasma.LPS stimulates their transportation from cytoplasm into nuclear.Cyclin D3 overexpression facilitates the accumulation of CDK11p58 in nuclear.Conclusion： LPS stimulates cyclin D3 and CDK11p58 expression in rat primary astrocytes.Cyclin D3 interacts with CDK11p58 to participate in the progression of astrocyte activation.