中文摘要：

分别在新城疫Ⅳ系弱毒苗中添加黏膜免疫佐剂乳酸杆菌、CpG DNA、重组IL-2、氟化钠和大豆黄酮，经口免疫鸡后，研究十二指肠、空肠、Peyer’s斑单位面积IgA分泌细胞的变化。首先提纯鸡IgA和制备兔抗鸡IgA血清，然后应用免疫组化技术显示鸡小肠IgA分泌细胞。结果表明，在免疫后第3周、第5周乳酸杆菌组比新城疫组（ND）极显著增加各段小肠IgA分泌细胞的数量（P〈0．01）；CpGDNA、重组IL-2和大豆黄酮在整个免疫期内均明显增加鸡小肠黏膜局部IgA分泌细胞数量；NaF对鸡体黏膜局部IgA分泌细胞数量无明显增加。结果表明乳酸杆菌、CpGDNA、重组IL-2和大豆黄酮是有效的口服黏膜免疫佐剂。

英文摘要：

In this paper we investigated effects of five kinds of mucosal immune adjuvant such as lactic acid bacillus（LAB）, CpG DNA, recombinant IL-2（rIL-2）, sodium fluoride（NaF） and daidzein, on IgA secreting cells of chicken small intestine （duodenum, jejunum and Peyer＇s patch）. Chickens of five groups were immunized orally with NDV strain LaSota vaccine containing different mucosal immune adjuvant metioned above respectively. And other two groups were given normal saline and LoSota vaccine as control. The results showed that the areas of IgA secreting cells of the Group LAB were increased significantly than that of Group ND （P〈0.01） at 3rd and 5th week after vaccination in small intestine. The areas of Group CpG DNA, rIL-2 and daidzein were increased during the whole immune period. No significant changes were found in Group NaF. The results demonstrated that LAB, CpG DNA, rIL-2 and daidzein were effective mucosal im mune adjuvants.