中文摘要：

分别应用复合黏膜免疫佐剂Ⅰ和Ⅱ与新城疫Ⅳ系弱毒苗混合（佐剂Ⅰ组和佐剂Ⅱ组）,经口免疫鸡后,研究鸡小肠黏膜局部CD3^＋T细胞、上皮内淋巴细胞和干扰素-γ（Interferon-γ,IFN-γ）mRNA表达水平的变化。结果表明,在整个免疫期内CD3^＋T细胞和上皮内淋巴细胞数量都呈上升趋势,在首免后第3和第5周时十二指肠CD3^＋T细胞佐剂Ⅱ组比佐剂Ⅰ组显著增加（P〈0.05）,空肠CD3^＋T细胞佐剂Ⅱ组比佐剂Ⅰ组极显著增加（P〈0.01）,第7周时Peyer’s斑上皮内淋巴细胞佐剂Ⅱ组比佐剂Ⅰ组极显著增加（P〈0.01）;整个免疫期内佐剂Ⅱ组和佐剂Ⅰ组IFN-γmRNA表达水平呈下降趋势,表达量较低。本研究表明2种复合黏膜免疫佐剂都能明显增加黏膜局部免疫细胞的数量,提高小肠局部黏膜免疫力。

英文摘要：

The chicken were immunized orally with NDV strain LaSota vaccine containing two kinds of compound mucosal immune adjuvant（Group adjuvant Ⅰ and Ⅱ） respectively.The CD3^＋T lymphocytes,intraepithelial lymphocytes（IEL） and IFN-γ mRNA expression of small intestine in chicken were investigated.The results showed that the number of CD3^＋T lymphocytes and IELs increased during the period of immunity.The number of CD3^＋T lymphocytes of Group adjuvant Ⅱ were higher than that of Group adjuvant Ⅰ（P〈0.05） in duodenum and significantly higher（P〈0.01） in jejunum at the 3rd week and 5th week after vaccination.The number of IELs of Group adjuvant Ⅱ were significantly higher than that of Group adjuvant Ⅰ（P〈0.01） in Peyer＇s Patch at the 7th week after vaccination.The IFN-γ mRNA expression of two compound adjuvant groups decreased after vaccination.The results demonstrated that compound adjuvants could induce high level cellular immune response and promote intestine mucosal immunity in chicken.